Measure Fluorescence intensity of whole spheroid

Hi community,

I am wondering if it is possible to take Z stack images of a spheroid, make a sum projection and measure the intensity of the spheroid. Similar to FACS but with a confocal microscope for the whole spheroid and not just single cells?

Is this bad bad practice because the spheroids are not comparable?

Do you have thoughts and ideas on this?
many thanks!

I don’t know that it’s bad practice, though how comparable the measurements are across images will definitely depend on your sampling density. It also depends on how your SUM projection works- if you end up maxing out the pixel values, you certainly won’t get a fully quantitative result.

Good luck!