Hi, I’m trying to measure a clacium wave on a series of images taken over time. The cells that I’m trying to measure are conected, so you can see a calcium wave propagation in the culture. I have been trying with roi manager to analyze fluctuations in each cell, but I have no idea how to measure a wave, to mark a start point, correlation of the response between two cells, etc.
Also, I was using "integrated density, since the cells have different sizes, but I saw in another post that I should use “mean grey value”, can you explain that please?
Thanks in advance!!!