Hi! I need to measure and get the independent measurement of the fluorescence signals of nucleolus, nucleus and cytoplasms of more than one cells in the same slice… the signals i have to measure is a green signal that localize in the different compartment of the cell… can anybody help me?
could you please upload some images where you want to do this quantitation?
have a nice day
IMG1.tif (60.5 KB)
thank you for answering
that’s the image I need to measure