Measure different roi

Hi! I need to measure and get the independent measurement of the fluorescence signals of nucleolus, nucleus and cytoplasms of more than one cells in the same slice… the signals i have to measure is a green signal that localize in the different compartment of the cell… can anybody help me?

dear Mazia,
could you please upload some images where you want to do this quantitation?

have a nice day
Emanuele

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IMG1.tif (60.5 KB)
thank you for answering
that’s the image I need to measure