I’m trying to identify cells that are at edges of clusters. My idea was to identify the cells, then expand the identified objects until adjacent cells touch each other, then use the MeasureObjectNeighbor to suss out cells that are on the border of clusters. However, the results from MeasureObjectNeighbor (in “Adjacent” mode) aren’t really what I expected. I expected the measurement “PercentTouching” to range from 0-100% (or 0-1) so that I could use that to filter out cells which did not touch other cells 100% (i.e. border cells). Is this a bug, or have I misunderstood how the PercentTouching measurement works? I’ve attached a screendump to show what I mean.
Also, is there a way to filter out expanded objects that touch the edge of the image?
Edit: I’m using CellProfiler version 11248.