Sample image and/or code
The image above is of fat cells that have been preserved with PFA then stained with H&E to see nuclei and cell walls.
I have been trying to measure fat cell volume using the Measure and Label macro (following Quantifying Size and Number of Adipocytes in Adipose Tissue by Parlee et al 2014) after having issues with Adiposoft. I process the raw image (Color Threshold-> binary) and you can see the resulting image above (black lines and white background).
When I run the Measure and Label macro it does a nice job of measuring the cell, however the label is incredibly small and white (can see in the red circle), so it makes its almost impossible to tell which ones I have already measured. I can’t figure out (nor have been able to find anything on) how to change the font size or color (changing the text tool font did nothing and I haven’t been able to change the measure and label macro as it is a .class extension) so that I can see the label. I also tried changing the color picker, but it always defaults to white/white when using the wand tool to select the cell I want to measure.
Thank you so much for your time and help,
PS here is an example of the original image: