Nature volume528 , pages280–285 (10 December 2015)
The part of image analysis in this paper, they detect signal positive particles using Gaussian fitting, which doesn’t rely on intensity threshold. It does not make any sense. Could you describe it, please? And how can I apply this technic to an image?
I attached an example image which has 3 z slices over time.![signal%20positive%20particle%20detection|690x385,50%](upload://tzK7mvsample(1).tif (11.3 MB)