We are following a fluorescent macromolecule uptake into lysosomes with using Integrated Density per cell.
At early times we have intensity values of Mean, Min, and Max such as 69, 27, and 190. And as time progresses these values increase, as expected.
But at the latest times, most of these values have Max values of 254, such as Mean, Min, and Max of 157, 49, and 254, or as in 78, 23, and 254. While these Max values are below 255 which I understand to be the maximum intensity possible, will these consistent 254 Max values indicate a “maxing out” of fluorescent intensity?
Thanks to any help!