Hi there, I have just started using cellprofiler and I was wondering if anyone can help me with a couple of aspects of the “CalculateMath” function?
Firstly, how do I export the results from the calculations as a spreadsheet? I have tens of images, with three CalculateMath commands per image, and I would like the results in a spreadsheet.
Secondly, is there a way to get a math result for each object? I have puncta, and I want it to calculate how many puncta each cell has, but at the moment I can only work out how to calculate the total number of puncta divided by the total number of cells to get the mean number of puncta per cell. I have tried dividing objects by other objects rather than images by images, and it doesn’t seem to do what I want to do.