I have images of cell where the nucleus and one protein is labeled. The protein in mostly in the nucleus except when I treat the cells with a drug, then the protein localizes to the periphery of the nucleus. I would like to quantify this by drawing a mask around the nucleus (they are not uniformly circular), measure the insensity of the signal, and then increasing the mask by 10% and taking a second measurement of the intensity of the signal.
Basically I would like to draw a mask and then dilate it.
How can I do this? What plug-ins would I need?
Any advice would be great!