Hi Guys (Mark),
I have a difficult case to segment, tips would be greatly appreciated!
Contained in the attached file are 5 axial images taken sequentially from a tif stack. My problem is that locally around each cell there is a uneven amount of noise. Sometimes the cell signal is weak and there is low background, sometimes the cell signal is high and there is high background (higher than the lower signal cells).
Any tips on how to best pre/ process these images for recognition?