I am looking for a solution to a problem that seems to reoccur in my CP analysis pipeline. I am trying to use “MeasureObjectIntensityDistribution” on my nuclei (ch0/DAPI). Instead of using the other channel image in which a significant amount of background occurs for the measurement, I’d like to measure only the spots in that channel, which I defined using IdentifyPrimaryObjects > MaskImage. However, when I choose the masked image as input, I am unable to get the measurement.
Example_pipeline.cpproj (1.7 MB)
- it used to work in previous pipelines and images, both using czi formats and tif.
- I ran other analyses in the meantime, which worked great.
- The pipeline I have constructed to show the issue here also contains the RadialObjectDistribution modules on channel images, which also work great.
Thanks so much for looking into this!