I have been using ImageJ with a macro plug-in to calculate leaf area for a variety of species. This has proven mostly effective, however, some scans have proven more difficult than others. That is, some scans are more difficult to black out than others (while processing the scans using macro plug-in). I’ve tried adjusting the threshold, however this isn’t producing the desired output. I’m hoping someone can provide a protocol or step-by-step instructions to manually calculate leaf area of these difficult scans.
Thanks for any and all help.