[quote=“mbray”]Glad to help! A few pieces of clarification:
(1) To save the objects as an image, use the ConvertToImage module with the dot objects as the input and binary as the color, followed by the SaveImages module
(2) To re-load the image, you can use the LoadSingleImage module, and then re-convert it into an object with IdentifyPrimAutomatic using 0.5 as the absolute threshold, ‘no’ for discarding/merging and ‘none’ for distinguishing clumped objects and for drawing dividing lines. This preserves the shape of the objects to be used as your template.
(3) At this point, you can use the MaskImage module if you want a masked image to use later, or if you are just looking for a measurement with respect to the input image, use a MeasureObject module using the object created from the dot template.
Thank you so much for the clarification. I have one more question. If I load many pictures for analysis, it seems I have to re-convert the template picture into an object with IdentifyPrimAutomatic for each picture. This step is acutally the most time-consuming process and it takes several minutes to identify one object on my laptop! Is there any way that I just need to re-identify the object once which could be used for the remaining pictures?