Manual segmentation possible


I have a set of confocal microscopy images of cells. The segmentation using Cellprofiler was not very accurate so is there a way

  1. to manually segment the cell membrane and nuclei using Cellprofiler
    and 2) use the manually-segmented image (Cellprofiler or other method) to use the ‘object processing’ or ‘measurement’ modules.

Yes, to both questions:

  • You can use the EditObjectsManually module to adjust the boundaries of a previously created object set, or IdentifyObjectsManually to create a new set of objects from scratch.
  • The objects edited/created by the above methods can be used in any of the object/measurement modules that take an object as input.