Dear CellProfiler users,
my name is Marco and I’m a newbie cellprofiler user, I’ve found problem in automatic identification of secondary objects. I’ve got images of confluent elongated adherent cells, the provided tool is quite precise in identification but sometime, due the particular cell morphology, it’s not able to correctly identify some cells. I’ve tried different threshold and post processing but things got just worse. Could you help me? Could be performed any manual modification of identified objects?
my pipeline (basic)
…different modules as enhanceEdges or Feature ect
PrimaryObjectId (for nuclei it’s always precise for correct tyoical diameter
SecondaryObjectId (quite precise with watershed-gradient, but I’d like to modify not correctly identified entities)
Images are attached