I need to make a z-stack from 2D images. I know how to make the z-stack but then there are a whole lot of settings that you need to get right if later you want to make a 3d model.
I have miscroscope images (they are all aligned) that are taken at 7 different focal points. I am guessing that I need to input pixel widht height and dept in order to make a 3d model? If so, how do I determine that? What about the distance between stacks?
I am really confused here and Im not sure where to start.