I have a set of images comprising 5 time points spanning a drug application (so that intensity of a fluorophor varies across time in response to drug), with 3 channels (nuclei, calcium, GAD). Timepoints 1 and 2 are designated “basal” and timepoints 3, 4 and 5 are designated “stimulated”. The objects are tracked through the timecourse to give them a unique ID. What I would like, for each object that I have segmented, is to be able to get the difference in intensity from the average of 1 & 2 (basal) to the average at 3, 4 and 5 (stimulated). Is there any way of doing this easily without having to go into the spreadsheet and try to manipulate the data?
Thanks so much for your time