I’m working on a project with nickel-based samples. I’m using the Focused Ion Beam (FIB) to analyze the samples slice by slice. My goal is to measure the density of a recreated 3D-model. In theory I want to:
1 - binarize every slice and determine the size of the etched precipitations
2 - recreate a 3D-model with the binarized images
At the moment I stuck at the “make binary” or “threshold” part. The images containing different grayscale’s, edges are highlighted and the inside of the etched areas are sometimes black, sometimes grey.
My first attempt was to correct the different grayscale’s in the image. I started with the SEM picture as following:
gp-001 - 001.tif (489.2 KB)
I used gaussian blur with a radius of 50 to divide the result with the SEM image and the result with the blurred one
gp-001 - 001_blur.tif (489.2 KB) is Result of gp-001 - 001.tif (489.2 KB).
But then every attempt to get a useful binary is not successful.
I would be very happy for any kind of advise since I’m not sure if its possible to work this out without marking every interesting section by hand.