I’m trying to analyze histological samples stained with a specific antibody, that brings a brown color (typical immunohistochemistry). In this case, renal tissue.
I found in the literature a protocol for easy quantification of this kind of images (Semi-quantitative Determination of Protein Expression using Immunohistochemistry Staining and Analysis: An Integrated Protocol - PubMed), and when I tried with one picture, it worked fine. The problem started when I aimed to produce a macro for an automatic (and quick) analysis of 140 images. When I recorded the procedure, it took the name of the image, that I don’t know how to eliminate from the macro, so that the program analyses all pictures of the input folder. In addition, after color deconvolution, only channel 2 is the correct one to measure, but I also don’t know how to select it, instead of channel 3.
Here is the macro I have after recording (sorry, I don’t know how to attach files here):
run(“Colour Deconvolution”, “vectors=[H DAB]”);
run(“Convert to Mask”);
I would appreciate it a lot if you can help me here. Maybe it’s only a tiny thing, but I’m unable to find the solution alone!