LSM channel separation problem

Hello!

We have some pictures in the LSM format and we can open these pictures with cell profiler, however when we use the module “color to grey” it seems that it cannot separate the different channels. When we convert the images to .tiff it seems to work.

Is there any way we can use the module in the LSM format in order to avoid converting a large amount of images to .tiff?

Thankful for all the help we can get!

Yes, but can you upload an example LSM file here? The metadata can take many forms, and we need to inspect to know for sure. You can zip the file if the Forum complains or use, say, Dropbox to host it.