I have a curious problem with my processing. My low-intensity 32 bit images are converted into high intensity values after a 16bit conversion. My normal-intensity values are fine to convert. Is anyone aware of a bug here? Is there a lower 32bit threshold that does something inverse to the data?
Below is an example of the results, where the negative control data are highlighted. It seems very specific for the low-intensity values, since the other values scale correctly.
Below is the image calculator macro I am running.
imageCalculator(“Subtract create 32-bit”, “RFP”,“Dark.tif”);
imageCalculator(“Divide create”, “RFPSub”,“MedianShade.tif”);
run(“16-bit”); // THIS IS A BUG IT MAKES THE NEGATIVE VALUES MUCH HIGHER AFTER 16 BIT CONVERSION
Any help would be much appreciated!