I’m designing a pipeline to analyze photos of human macrophages and fibrocytes, two closely related cell types in the same population.
I’d like to be able to design a pipeline that
A) Identified macrophages, then
B) Removes the macrophages from further analysis and
C) Allows me to identify the remaining fibrocytes with another “IdentifyPrimaryObjects” module.
In short, I want to identify macrophages, tell cell profiler not to include macrophages in a subsequent analysis, and then use the resulting image to identify fibrocytes.
Then I can figure out the staining intensity for each cell type.
I’ll upload a sample image and the pipeline I’ve constructed.
I need a module that let’s me discard objects that meet previously defined parameters.
Thanks very much,
adjusted filter objects.cpproj (240 KB)