Dear ImageJ community,
I’m facing a little limitation in imageJ analysis.
I’m trying to threshold the following image. In this image I have very bright spots (in the red square) and very faint ones (in the white square - that I can only see when I increase my image brightness balance).
-> My objective is to threshold in the same image the bright and dim spots.
The bright ones are easy to threshold but to get the dim ones it’s another story because if I use a general threshold like Huang I can get the dim spots but then I loose the size of the bright ones that appear bigger.
I usually use a local threshold to solve this problem, like the Bernsen but this time it doesn’t work, as you can see. And I tried to play with all the parameters of the local threshold without much success.
Does anyone know a better local thresholding method with imageJ?
Thanks a lot in advance for your help.
Have a good day.