For a project I’m assisting on, I need to get object data from the nuclei for both Hoechst and BRDU, respectively, and then a count of BRDU positive cells compared to the Hoechst nuclei (since the BRDU positive nuclei are also in that image). The raw images from another grad student in my lab come in sets of 2: one with Hoechst and a second with BRDU with the same field of view. I have two images an an example of the set (00 for Hoechst and 01 for BRDU). To make life a little easier I used imageJ to try to make a composite TIFF file so only one file with 2 channels need be loaded in the pipeline. However, I keep getting a “list index out of range” error when I’m in test mode and with some considerable time testing different ways to upload the images I can’t seem to make the pipeline work. Can anyone lend some assistance in this matter? I would be greatly appreciative.
Nucleus- 40x objective-- BRDU.cp (13 KB)