I was wondering if I could get some advice on how to upload files for 3D analysis. My images were originally in czi format, which I got to know is not supported for 3D processing in CellProfiler 3, so I exported them as tiff files. I have 4 channels and varying amount of z-stacks for each image which ended up being exported as individual images of one stack of one channel. However, when I tried to process my files in Watershed I got an error message saying my array and my Bc has different coordinates. I had no idea what is a Bc and how to solve this issue, but I suspected it might be due to my files not being actual stacks any more. I read a few more threads in the forum here and tried to change my image to contain all the stack for each channel and have 4 instead of 32 images. But now, I’m not even able to update my NamesAndTypes section.
Could you please advise me on what exactly am I doing wrong? Thank you very much for the help.
I have attached my project file and my test image.
2.7_LMNA_Nucl_HA-KEKS_a_c1_ORG.tif (8.0 MB) 2.7_LMNA_Nucl_HA-KEKS_a_c2_ORG.tif (8.0 MB) 2.7_LMNA_Nucl_HA-KEKS_a_c3_ORG.tif (8.0 MB) 2.7_LMNA_Nucl_HA-KEKS_a_c4_ORG.tif (8.0 MB)
Nucleolus count project.cpproj (191.3 KB)