Live Imaging, Measuring Endothelial Gaps


I am trying to measure gaps IN BETWEEN THE CELLS in live imaging. So I will have a time series of 2D images with dynamic formation and closure of gaps in between the cells and I’d like to measure these with cell profiler.

I am using a membrane stain( WGA 633) as well as F-actin which should clearly dilineate the cell border, BUT I will NOT be imaging nuclei…

Do you have any suggestions on how I can do this?