I’ve been trying to analyze lipid droplets in cells stained with Hoechst and Lipidspot (red channel), using the suggested pipeline on the website. However, when I observe the lipid droplets identification quality using edit objects manually, I see that not a lot of them are recognized when the guiding image is masked LD using nuclei objects.
I was wondering if anyone has any ideas about how to improve it?
Attaching all the relevant images and the pipeline here.
001__cntrl 1-3C_cntrl1a - try1.tif (12.0 MB)
pipeline try3.cpproj (919.4 KB)
Thanks a lot,