I have recently optimized a pipeline for the analyzis of lipid droplets in mammalian cells. In the protocol, cells are stained by DAPI, Bodipy and Phalloidin to visualize the nuclei, the lipid droplets and the cytoplasm, respecively. As I don’t have many experiences in image analysis, I would like to ask your opinion about the enclosed pipeline. Which modules, settings should be changed? Unfortunately one cycle of image analysis (one image set) on my personal computer takes around 4-5 min. Do you have any suggestions to reduce it?
PS: in the attachment you can find an representative image set, the pipeline, a dtabase and properties file.
Test pipeline 0308.rar (3.93 MB)