I’m having a problem understanding how to link tables together in the Analyst tool.
I have trained the classifier to recognize two different cells populations (positive or negative) and scored all of my images for these phenotypes. Now that I have done this, I would like to use the scatter plot, histogram, and heatmap features to examine per object measurements for each of my classes (positive or negative). I want to do this to see (and show) the important measurements for defining my phenotypes.
My problem is that my ‘HitTable’ (created from scoring all images after training) is not linked to the per_objects table, and I have no clue how to achieve this. I have tried many different variations of linking using the “OTHER TABLE” option in the drop down menus in the heatmap/scatterplot options, but none seem to work for me. Perhaps I am missing a step somewhere?
Also, a minor problem I have observed is that when you are setting a gate in the heatmap/scatterplot options on some data filtered by plate/well/ image, and you request to see the cells in that gate, the filters do not apply to the cells you are shown. i.e. when I filter by my mutant and request cells in a gate, the program will show me cells from my wild-type images too. Perhaps I am missing something here too?
Thanks for any help you can provide.