Lines not remaining permanent

Hi all,
I’m having trouble getting a line to be drawn in permanently for a signal slice. I’ve used the Draw tool to make it permanent, scrolled to the next frame and back, and it is no longer on that slice. Any thoughts on how to fix this?

I think we need more background information which file format do you use, which OS and how you open the stack.

However my guess is that you are opening the stack as a Virtual Stack where changes get lost:

https://imagej.nih.gov/ij/docs/guide/146-8.html

…Virtual stacks are read-only, so changes made to the pixel data are not saved when you switch to a different slice…

2 Likes

Thank you very much, we realized that if the Virtual Stack box is checked then the stack will act as a read only. Your help was much appreciated!