I want to use the 3D viewer of Fiji ImageJ to take a rotation video of a rekonstructed cone like object. The problem is, that the slices have already been normalized seperatly, so that the brightness of the background is not the same for all images. At the top of the cone, the background is as bright, as the material at the bottom of the cone. So I cannot adjust a threshold to represent the object.
Is it possible to reduce the brightness linearly through the stack? Or do you have an alternative idea.