Limitations of using QuPath in H-DAB protein quantification

Hi there,

Can anyone suggest the limitations of using QuPath in quantifying the expression of an intracellular protein in DAB stained tissues? I know that DAB staining doesn’t correlate to linear protein quantification, but can anyone think of any other limitations?

Thank you :slight_smile:

Is this a duplicate of your earlier question? Limitations of Qupath - #4 by Dhylan

If you aren’t satisfied with the very good answers already provided there, I would suggest adding more specific information on the original topic. It’s really impossible to give a thorough answer without seeing some images and better understanding the reason why you are asking, and reposting a similar topic is unlikely to lead to better responses.