I’m trying to determine the fraction of overlapping pixels between two channels. I thresholded my two images, and generated a new image with just the overlapping pixels using the image calculator. Now I want to get the area or pixel number from each image but something weird is going on.
I’m just trying to get an area measurement of the white pixels by doing Analyze>Measure after having check the “limit to threshold” box in measurement settings. But it is just giving the area of the whole image, can anyone tell me what I’m doing wrong?