I am unable to find a file format description for Leica lif file. Is there any documentation for it? I am especially curious in FLIM and FCS part.
Hi @Piter, the Bio-Formats team don’t have any documents which we are allowed to distribute but if it helps we have some public sample files at https://downloads.openmicroscopy.org/images/Leica-LIF/, these can be accessed via the Bio-Formats Importer or LAS X software (can be downloaded from https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/)
If you really want to dig deep into our LIF Reader to see the internal structure then the source code is at https://github.com/ome/bioformats/blob/c29e626296fcf360da9959eaec041a7d2c885f69/components/formats-gpl/src/loci/formats/in/LIFReader.java
I will have a look but to my shame I am java illiterate. also to my knowledge bioformat does not read FlIM and FCS data. Images I can read from lif in python, I was hoping to get insight on how time resolved data stored. For now I go with ptu export.