I’m fairly new at Fiji, but I have to process thousands of pictures for leaf area measurements, taken with different focal distances. They also have shadows and the leaves are placed over a graph paper (light background, but not white). Well, I’ve been calculating the areas by hand, photo by photo, since I have to set the scale manually for each photo. I was wondering if there is a more automatic and less consuming time for doing this. I thought that maybe I could interpolate the images, and so I wouldn’t need to set the scales manually - but I don’t know how and if it’s appropriate to do it ( and also I’d still have to deal with the shadows).
Anyway, I’ll appreciate any thoughts, or tips, on the matter!