I have to measure maximum anterior-posterior length of bones (condyles) in X-ray images of knee. I am using ImageJ (Fiji) software for that. However, one problem is that I have to incorporate the phantom of 30mm in the readings as well. How I can do scaling for that in ImageJ?
Hi @sajia and welcome to the forum!
first, you need to make sure that the spatial calibration (a.k.a pixel size, e.g. mm/px) is properly set in your image. You can
check if the image is calibrated in
Image > Properties, and if necessary
set the spatial calibration using
Analyze > Set Scale
then you can insert a scale bar using
Analyze > Tools > Scale Bar
Hope this helps!
Hey Thanks alot for your reply.That helped alot! I am new using ImageJ so pardon me if I may ask how can I get all 3 views (coronal, sagittal and axial view) in DICOM data…I have tried stack<<orthogonal views but I can’t get desired orientations and even slider to adjust all 3 views.
if you have volumetric data, then orthogonal views will give you the 3 views, but the axis XYZ will always be with respect to how the data was recorded.
You have a bit more control using
Image > Stacks > Reslice , but if your sample axis (say “coronal”) is not aligned with the microscopy axis, then you will have to rotate or even translate the image beforehand using
Image > Transform > Rotate and
Image > Transform > Translate