Hi Cell Profiler Team,
I just started with cell profiler and try to figure out a pipeline for following problem:
I have co-culutures where one cell type is stained with Quantum dots (QT705) to distinguish the cells, furthermore the cells are stained with DAPI and a KI67 (Alexa488) antibody to identify proliferating cells. Now I like to know how
- how many cells are in my picture (DAPI)
- how cells are Quantum dot positive
- how many cells are stained with Ki67, how many of these KI67 stained cells are stained with Quantum dots.
With the attached pipeline I identify always as many DAPI positive cells as labeled cells (which is not true). I might be that I did not choose the perfect threshold and identification method for the Quantum dots since there dotted structure is not easy to identify for the programm. The identification of the KI67 seems to work (just needs optimisation for the threshold).
Hope you can help me solving my problem. Thanks in advance!
DefaultOUT__7.mat (219 KB)