So I just downloaded ImageJ for a new job I am working on. They required me to get the ITCN plugin to get nuclei counts on muscle fibers. However their protocol includes adjusting a threshold value which is no longer an option when using the plugin. Since there doesn’t seem to be a point of contact for the software and we can’t seem to find it anywhere else I was hoping that someone here might know what I could do or how I could get access to that threshold option again?
Any help would be greatly appreciated!!