I am using the ITCN plugin to perform automated nuclei counting. I have been having problems with deciding what parameters to use. I also am unsure what unit of measurement the plugin is asking for (pixels or um). Most of the time I try to use the plugin there is an error message when I add my parameters. I believe I may be having problems with image scale. I successful ran the plugin in once but the total count it generated was rather unrealistic; therefore, I’m still doing something wrong. Is there any way someone could include step by step instructions on how to use the plugin so that I can continue to troubleshoot?
Thanks for your help and feedback.