I am trying to perform colour deconvolution, but when I convert myimage to binary, I am unable to manually adjust the threshold to accurately differentiate cell bodies. As soon as I adjust the scale bar the image ether goes completely white or black. The first image is immediately after I make binary, the second when I attempt to adjust threshold.
Has anyone had this issue before? I have tried updating my computer (mac), updating image J, using a previous version of image J, and also trying on my windows PC, to no avail. Any advice would be amazing!