I’m new to this online space, however I intend on immersing myself further in this community as my research has required the basic uses of ImageJ for a few years. However I have been increasing the complexity of my ImageJ usage and I have encountered some issues. If someone can assist me, it would be appreciated.
As a preface, I am using a Mac, and running the most recent software (Catalina 10.15.x). My microscope photos are being taken on an AmScope MU500, using the AmLite software. I have been saving my photos as .tif and .tiff. Additionally, I have very little experience with coding.
I’ve been attempting to stitch together my photos to give a large and high-resolution images for analysis.
I have strictly followed the steps outlined on the Stowers Institute for Medical Research on YouTube (https://www.youtube.com/watch?v=S54udQGGcS4). Yet I haven’t been able to stitch together my images and have received this error:
Stitching internal version: 1.2
**Loading (0, 0): /Users/myname/Desktop/Tif test/tile_1.tiff … **
ERROR: Cannot find file: ‘/Users/myname/Desktop/Tif test/tile_1.tiff’ - abort stitching.
ERROR: Error during tile discovery, or invalid grid type. Aborting.
I have tried “BigStitcher” and “MIST” with different issues.
Any assistance would be greatly appreciated as I’ve put in a significant amount of time trying to fix this, but with no success.