I\m new to this amazing software. However, i\m having some issues to identify and measure pupae of flies. There is a successful example of identifying pupae here, in that topic the pipeline is also attached. Please see here Extending the use of WormTools to Drosophila Pupae
But idon\t know why this pipeline does not fit my images. It would be great if someone here could help me figure out. The image is attached.
ps, The purpose of my study is to identify all pupae and measure the size (area or height), and rank them according to the measurements. I know that it could be able to measure and show the data on image, but do we also have a ranking function, where enable the image only display the biggest ones?
ps, the image is taken by cellphone under the lights. We can easily find the shade, will this affect the measurement and how can we remove?
Best from Long