I have some issues with the processing of some images. I have like 100/200/500/1000 or more pictures per condition (temperature), and I have several for different materials hence I have a lot. I observe crystallization, so I decide to analyze an individual crystal instead of the collection that I have on the full picture. I would like to get the particle size evolution for each condition and much parameters as I can.
I process the images with the following options. The main problem is using Threshold.
run(“Gaussian Blur…”, “sigma=2”);
run(“Enhance Contrast…”, “saturated=3 normalize”);
run(“Convert to Mask”);
run(“EDM Binary Operations”, “iterations=1 operation=dilate”);
run(“EDM Binary Operations”, “iterations=2 operation=close”);
run(“EDM Binary Operations”, “iterations=3 operation=open”);
run(“EDM Binary Operations”, “iterations=3 operation=erode”);
run(“Analyze Particles…”, “size=10-Infinity display exclude clear summarize add in_situ”);
However, it works just for one image and not for the remaining, also I have tried other options like e.g substrate background, weka segmentation or morphological segmentation, any suggestion is welcome!
For some reason, I can not upload my images. Take a look at the link please: https://drive.google.com/drive/folders/1o1hM1V08KbmbzIkffkEpMRtWu_gOSdxg?usp=sharing