I have a question about the objects numbers. And here is what I do:
- I load to image sequences (timelapse) and identify objects in a combined image calculated from the respective frame of the 2 input sequences
- then the object intensities in both frames are measured and the ratio is calulated
The question I have, is the following. When I display the detected objects with the object numbers as an overlay, the number for a certain object changes sometimes from frame to frame. But my purpose is to track the ratio of a ceratin object over time and when in sort the resulting excel sheet afterwards for object xy (to plot it), this may not be the identical cell for all frames, since in most of the frames the object number for this idividal cell(as an example) is 20, but once a while it might be 19 or 21?
I attached the pipeline and a movie of the detected ROIs with the object numbers to make my point more clear.
Thanks for your help,
Ratio340_380_TILL_display.cp (8.46 KB)