I’m trying to learn how to do 3D segmentation in CellProfiler, so I’ve been working through the tutorial on GitHub (https://github.com/CellProfiler/tutorials). I can get through everything ok, but when I try to open my saved images with overlays, FIJI gives me an error: java.util.zip.DataFormatException: incorrect header check
If I save my images as 8-bit tiffs, it works ok, but I’d like to be able to save them as 16-bit. Are there particular settings I need? When I take this image and read it back into CellProfiler, it works fine, so it must be something about FIJI specifically.