Here I have two difference strains of cell that qualitatively seem to have different patterns of signal. The top one, “nap,” seems to demonstrate its signal more diffusely and has thinner stretches of actin (signal), whereas the bottom one, Wild-type, has blockier signal more localized toward the middle of the cell. This is a recurring trend that my lab has observed, not just in these two cells.
How do I quantify this difference? I feel like I need to be able to quantify the SD of the signal relative to the center of the cells mass, yet Im not sure how to set this up. My search has been a bit futile so far. Anyone have any ideas?