Hi, I have an image of ki67 cells that I really need help with. I have been able to count the total number of nuclei in the image using cellprofiler by inverting the image and then asking it to count the total number of nuclei by using primautomatic.
However, as you see in the image, there are brown and gray nuclei. Is there a way for cellprofiler to pick out just the brown nuclei and give me that number?
There’s the link to the picture.