I’m trying to quantify cell spreading of MSCs on a 2D surface. Pictures were taken with a phase-contrast microscope and I want to figure out the cell adhesion kinetics. Are there any software/plug-ins that can quantify the cell spreading if I feed say a TIFF or .avi time lapse stack of images into it?
The general approach would be to segment the cells then measure each object’s area. For segmentation of phase contrast images, you may want to try ilastik, the trainable weka segmentation or MorphoLibJ, all available as plugins for fiji. If you provide more details, someone may come up with a more detailed solution.