Is Leica TCS LSI macroconfocal compatible with micro-manager?

Hello,

We have a leica TCS LSI macroconfocal in the lab (https://downloads.leica-microsystems.com/Leica%20TCS%20LSI/Brochures/Leica_TCS_LSI-Brochure_Technical_Data.EN.pdf). The version that we have does not have a camera. It works very well for now as a confocal system, but I would like to add a camera on it to have the possibility to do widefield Z stacks of fluorescent samples. Unfortunately the LAS software controlling it is outdated, does not allow the control of a camera, and updates to newer versions of LAS will apparently not be compatible with the hardware according to Leica.

So I am wondering if micromanager would work. I have no experience with micromanager so far, but I could see that a lot of the Leica hardware seems to be compatible. However I didn’t find any information about the super Z galvo stage. Does anyone have experience with this and may know if we will be able to use fully our Leica TCS LSI system with micromanager?

I am also wondering if having LAS and micromanager installed in parallel (although not running in parallel) may cause any conflicts or irreversibly alter our current set up?

Thanks,

Stéphane

Leica has been pretty good about making their microscopes compatible with their DMI/DMR protocols, so you could just try to run Micro-Manager on the computer currently controlling the microscope and see if MM can communicate with the scope. If that does not work, you can ask Leica for documentation to the interface between computer and microscope and find a programmer to help you write a Micro-Manager device adapter.

I am also wondering if having LAS and micromanager installed in parallel (although not running in parallel) may cause any conflicts or irreversibly alter our current set up?

There should be no conflict. Micro-Manager never “writes” settings to equipment, so unless something very strange happens (don’t think I ever heard about such a thing), all should be fine.

Great! Thanks for the very quick reply!
Then as you suggest I think I’ll try in the coming weeks and see if it works!