From the images I have, am interested in specific cells. I want to generate nucleus objects and use them to propagate and identify the cytoplasm using IdentifySecondaryObjects module in Cellprofiler.
I can obtain XY centroids for the nuclei, but I would like to know if there is an option in CellProfiler or ImageJ which allows creating objects using XY coordinates.
I appreciate a lot of any suggestions.
Thank you all,